• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> PARP Antibody

PARP Antibody (R31722)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R31722 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
›
IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: PARP antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of PARP antibody and A549 cells
Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320, 4) Jurkat, 5) rat PC-12, 6) mouse NIH 3T3 and 7) mouse HEPA 1-6 lysate with PARP antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa (full length), ~89 kDa (C-terminal catalytic domain).
IHC-P: PARP antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 142
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This PARP antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB
    Reactivity : Human
    Rab Mono Image
  • Applications : WB
    Reactivity : Human
    Rab Mono Image
  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine
  • Applications : WB, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse

Description

Poly [ADP-ribose] polymerase 1 is composed of four domains: a DNA-binding domain, a caspase-cleaved domain, an auto-modification domain, and a catalytic domain. In vivo, PARP is cleaved by caspases -3 and -7 between aspartic acid 214 and glycine 215, resulting in a 24KD subunit (containing the DNA binding zinc finger motif) and a 89KD subunit (containing the catalytic and auto-modification domains).
PARP is an enzyme that modifies nuclear proteins. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PARP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 670-858) was used as the immunogen for this PARP antibody.

Storage

After reconstitution, the PARP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all PARP antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.