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Home >> Antibodies >> PANX1 Antibody / Pannexin 1

PANX1 Antibody / Pannexin 1 (RQ7295)

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  Catalog No Formulation Size Price (USD)  
Image RQ7295 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with PANX1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with PANX1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human MCF7 cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
Flow cytometry testing of human SiHa cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse NIH 3T3 cell lysate with PANX1 antibody. Predicted molecular weight ~48 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96RD7
Localization Cytoplasm, cell membrane, cell junction
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PANX1 antibody is available for research use only.
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Description

Pannexin 1 is a protein in humans that is encoded by the PANX1 gene. The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties.

Application Notes

Optimal dilution of the PANX1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-D362) was used as the immunogen for the PANX1 antibody.

Storage

After reconstitution, the PANX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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