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Home >> Antibodies >> p73 Antibody

p73 Antibody (F51407)

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  Catalog No Formulation Size Price (USD)  
Image F51407-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F51407-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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p73 antibody western blot analysis in 1) human K562 cell line and 2) mouse brain tissue lysate.
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p73 antibody western blot analysis in 1) human K562 cell line and 2) mouse brain tissue lysate.
p73 antibody IHC analysis in formalin fixed and paraffin embedded mouse brain.
Confocal immunofluorescent analysis of p73 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
p73 antibody flow cytometric analysis of 293 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • p73 antibody western blot analysis in 1) human K562 cell line and 2) mouse brain tissue lysate.
  • p73 antibody IHC analysis in formalin fixed and paraffin embedded mouse brain.
  • Confocal immunofluorescent analysis of p73 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
  • p73 antibody flow cytometric analysis of 293 cells (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="p73 antibody flow cytometric analysis of 293 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O15350
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This p73 antibody is available for research use only.
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Description

TP73 is tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.

Application Notes

Titration of the p73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 288-317 from the human protein was used as the immunogen for this p73 antibody.

Storage

Aliquot the p73 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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