p65 Antibody / NF-kB (R30754)

Description

RELA, also called NFKB3 or NFKB, p65 subunit is part of the NF-kB complex. The complex is inhibited by I-kappa-B proteins, which inactivates NFkB by trapping it in the cytoplasm. The p65 heterodimer is the most abundant form of NF-kB. It is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NF-kB responses. Its nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on Ser468 enhanced binding of GCN5 to RELA and its ubiquitination.

Application Notes

The stated application concentrations are suggested starting points. Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. HIER: steaming with pH6 citrate buffer is required for FFPE testing.

Immunogen

An amino acid sequence from the middle region of the human protein (VPIEEQRGDYDLNAVR) was used as the immunogen for this p65 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the p65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.