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Home >> Antibodies >> p65 Antibody (RELA)

p65 Antibody (RELA) (F49893)

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  Catalog No Formulation Size Price (USD)  
Image F49893-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49893-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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p65 antibody western blot analysis in (1) HeLa, (2) MCF-7, (3) Raji and (4) Ramos lysate.
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p65 antibody western blot analysis in (1) HeLa, (2) MCF-7, (3) Raji and (4) Ramos lysate.
IHC analysis of FFPE human colon carcinoma stained with p65 antibody
p65 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of p65 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Chicken, Mouse, Xenopus
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q04206
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This p65 antibody is available for research use only.
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Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. [UniProt]

Application Notes

Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 10-37 from the human protein was used as the immunogen for this p65 antibody.

Storage

Aliquot the p65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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