p27 Antibody [clone DCS-72.F6] (V2437)

Description

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Application Notes

Optimal dilution of the p27 antibody to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Mouse recombinant protein was used as the immunogen for the p27 antibody. The epitope has been localized to amino acids 83-204.

Storage

Store the p27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).