• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> p-Rb1 Antibody (pS811)

p-Rb1 Antibody (pS811) (F48447)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F48447-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48447-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
Confocal immunofluorescent analysis of p-Rb1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
›
Confocal immunofluorescent analysis of p-Rb1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Dot blot analysis of p-Rb1 antibody. 50ng of nonphos-peptide or phos-peptide were adsorbed on their respective dots.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the p-Rb1 antibody.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P06400
Applications Dot blot : 1:500
IHC (Paraffin) : 1:50-1:100
Immunofluorescence : 1:10-1:50
Limitations This p-Rb1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of Rb1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a p-Rb1/HDAC1 repressor complex. Upon calcium influx, Rb1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of Rb1/E2F1 complex thereby disrupting Rb1's activity. [UniProt]

Application Notes

Titration of the p-Rb1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This p-Rb1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS811 of human Retinoblastoma.

Storage

Aliquot the p-Rb1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all p-Rb1 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.