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Home >> Antibodies >> P-Cadherin Antibody / CDH3

P-Cadherin Antibody / CDH3 [clone 6A9] (V3397)

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  Catalog No Formulation Size Price (USD)  
Image V3397-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3397-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3397SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human A431 cell lysate with P-Cadherin antibody (clone 6A9). Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated).
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Western blot testing of human A431 cell lysate with P-Cadherin antibody (clone 6A9). Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free P-Cadherin Antibody (clone 6A9). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 6A9
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P22223
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Limitations This P-Cadherin antibody is available for research use only.
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Description

Recognizes a protein of 116kDa, identified as P-Cadherin, or Cadherin 3 or CDH3. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the P-Cadherin antibody to be titered up or down for optimal performance.

Immunogen

Membrane from trypsinized A431 cells was used as the immunogen for this P-Cadherin antibody.

Storage

Store the P-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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