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Home >> Antibodies >> OPN Antibody / Osteopontin

OPN Antibody / Osteopontin (R30503)

  Catalog No Formulation Size Price (USD)  
Image R30503 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human HepG2 cell lysate with OPN antibody. Predicted molecular weight ~35 kDa but may be observed at higher molecular weights due to glycosylation.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P10451
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Limitations This OPN antibody is available for research use only.
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Description

Osteopontin is also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrcellular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the OPN antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human Osteopontin (EEKQLYNKYPDAVATWLNPD) was used as the immunogen for this OPN antibody.

Storage

After reconstitution, the OPN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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