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Home >> Antibodies >> Nucleolin Antibody

Nucleolin Antibody [clone 130CT43.8.5] (F40212)

  Catalog No Formulation Size Price (USD)  
Image F40212-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40212-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
Confocal immunofluorescent analysis of Nucleolin antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 130CT43.8.5
Purity Purified
UniProt P19338
Applications Western blot : 1:500-1:1000
IHC (Paraffin) : 1:50-1:100
Immunofluorescence : 1:10-1:50
Limitations This Nucleolin antibody is available for research use only.
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Description

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

Application Notes

Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This Nucleolin antibody was produced from mice immunized with a protein from human NCL.

Storage

Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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