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Home >> Antibodies >> Nucleoli Marker Antibody

Nucleoli Marker Antibody [clone NCM102-1] (V7035)

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  Catalog No Formulation Size Price (USD)  
Image V7035-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7035-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7035SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7035IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of Nucleoli Marker antibody and FFPE human tonsil tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC testing of Nucleoli Marker antibody and FFPE human tonsil tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of AF488 conjugated Nucleoli Marker antibody and FFPE human colon carcinoma. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Intracellular FACS testing of human 293 cells with Nucleoli Marker antibody (green) and isotype control (gray).
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NCM102-1
Purity Protein G affinity chromatography
Localization Nucleoli
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Nucleoli Marker antibody is available for research use only.
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  • Applications : FACS, IF, ICC, IHC-P
    Reactivity : Human; does not cross with rat
    Citation  Citations(2)

Description

The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

Application Notes

Titering of the Nucleoli Marker antibody may be required for optimal performance.

2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Storage

Store the Nucleoli Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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