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Home >> Antibodies >> NR1H4 Antibody / FXR (C-Terminal Region)

NR1H4 Antibody / FXR (C-Terminal Region) (R32869)

  Catalog No Formulation Size Price (USD)  
Image R32869 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IF/ICC staining of FFPE human A549 cells with NR1H4 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HCCT and 2) HCCP cell lysate with NR1H4 antibody at 0.5ug/ml. Predicted molecular weight ~54 kDa.
Flow cytometry testing of human A549 cells with NR1H4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NR1H4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q96RI1
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This NR1H4 antibody is available for research use only.
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Description

The bile acid receptor (BAR), also known as farnesoid X receptor (FXR) or NR1H4 (nuclear receptor subfamily 1, group H, member 4) is a nuclear receptor that is encoded by the NR1H4 gene in humans. This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport.

Application Notes

Optimal dilution of the NR1H4 antibody should be determined by the researcher.

Immunogen

Amino acids 442-486 (QHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ) were used as the immunogen for the NR1H4 antibody.

Storage

After reconstitution, the NR1H4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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