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Home >> Antibodies >> NOXA1 Antibody / NADPH oxidase activator 1

NOXA1 Antibody / NADPH oxidase activator 1 (R31030)

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  Catalog No Formulation Size Price (USD)  
Image R31030 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of NOXA1 antibody and Lane 1: U87; 2: HeLa cell lysate. Predicted/observed molecular weight ~51 kDa.
Western blot testing of NOXA1 antibody and Lane 1: U87; 2: HeLa cell lysate. Predicted/observed molecular weight ~51 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q86UR1
Applications Western blot : 0.5-1ug/ml
Limitations This NOXA1 antibody is available for research use only.
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Description

NADPH oxidase activator 1, also called NOX Activator 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the gene to chromosome 9q34.3 based on an alignment of the protein sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, but not GDP-bound RAC1. It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of NOXA1 was required for these interactions. Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the NOXA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human NADPH oxidase activator 1 (RQVPRGEVFRPHRWHLKHLE) was used as the immunogen for this NOXA1 antibody.

Storage

After reconstitution, the NOXA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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