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Home >> Antibodies >> NOX4 Antibody / NADPH Oxidase 4

NOX4 Antibody / NADPH Oxidase 4 [clone NOX4/1245] (V3410)

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  Catalog No Formulation Size Price (USD)  
Image V3410-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3410-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3410SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free NOX4 Antibody (clone NOX4/1245). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free NOX4 Antibody (clone NOX4/1245). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name NOX4/1245
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt Q9NPH5
Gene ID 4821
Localization Cytoplasmic and cell surface
Applications ELISA (order BSA/sodium azide-free format for coating) :
Western blot : 0.5-1ug/ml
Limitations This NOX4 antibody is available for research use only.
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Description

The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Application Notes

Titering of the NOX4 antibody may be required for optimal performance.

Immunogen

Human recombinant protein was used as the immunogen for this NOX4 antibody.

Storage

Store the NOX4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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