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  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant NKX2.2 Antibody / Rabbit Monoclonal

Recombinant NKX2.2 Antibody / Rabbit Monoclonal [clone RMNK2-1] (V7226)

  Catalog No Formulation Size Price (USD)  
V7226-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 379

V7226-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V7226SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 399

V7226IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 379
IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Species Reactivity Human. Other species not known.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, k
Clone Name RMNK2-1
Purity Protein A affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 4821
Localization Nuclear
Applications Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant NKX2.2 antibody is available for research use only.

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Description

NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]

Application Notes

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Storage

Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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