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Home >> Antibodies >> NFATC4 Antibody

NFATC4 Antibody (R32926)

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  Catalog No Formulation Size Price (USD)  
Image R32926 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat testis, 2) mouse testis, 3) human HeLa, 4) human A549 and 6) human SK-OV-3 lysate with NFATC4 antibody at 0.5ug/ml. Predicted molecular weight ~95 kDa, also can be observed at 120-140 kDa.
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Western blot testing of 1) rat testis, 2) mouse testis, 3) human HeLa, 4) human A549 and 6) human SK-OV-3 lysate with NFATC4 antibody at 0.5ug/ml. Predicted molecular weight ~95 kDa, also can be observed at 120-140 kDa.
Flow cytometry testing of human U-2 OS cells with NFATC4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFATC4 antibody.
Flow cytometry testing of human SiHa cells with NFATC4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFATC4 antibody.
Flow cytometry testing of human A431 cells with NFATC4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFATC4 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q14934
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA (recombinant human protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This NFATC4 antibody is available for research use only.
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Description

Nuclear factor of activated T-cells, cytoplasmic 4 is a protein that in humans is encoded by the NFATC4 gene. This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4.

Application Notes

Optimal dilution of the NFATC4 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids Q413-D522 was used as the immunogen for the NFATC4 antibody.

Storage

After reconstitution, the NFATC4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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