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Home >> Antibodies >> NFAT4 Antibody / NFATC3

NFAT4 Antibody / NFATC3 (R32932)

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  Catalog No Formulation Size Price (USD)  
Image R32932 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 22RV1 cell lysate with NFAT4 antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa but can be observed at 140~150 kDa due to phosphorylation.
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Western blot testing of human 22RV1 cell lysate with NFAT4 antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa but can be observed at 140~150 kDa due to phosphorylation.
Flow cytometry testing of human SiHa cells with NFAT4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human U-2 OS cells with NFAT4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human K562 cells with NFAT4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human A431 cells with NFAT4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q12968
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA (recombinant human protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This NFAT4 antibody is available for research use only.
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Description

Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.

Application Notes

Optimal dilution of the NFAT4 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids K630-L712 was used as the immunogen for the NFAT4 antibody.

Storage

After reconstitution, the NFAT4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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