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Home >> Antibodies >> NDUFB7 Antibody

NDUFB7 Antibody (RQ8099)

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  Catalog No Formulation Size Price (USD)  
Image RQ8099 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human tonsil tissue with NDUFB7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human tonsil tissue with NDUFB7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human skin squamous carcinoma tissue with NDUFB7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with NDUFB7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF7, 2) human PC-3, 3) human 293T and 4) mouse brain tissue lysate with NDUFB7 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of fixed and permeabilized human U937 cells with NDUFB7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFB7 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P17568
Localization Cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NDUFB7 antibody is available for research use only.
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Description

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, also known as complex I-B18, is an enzyme that in humans is encoded by the NDUFB7 gene. The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Application Notes

Optimal dilution of the NDUFB7 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-R98) was used as the immunogen for the NDUFB7 antibody.

Storage

After reconstitution, the NDUFB7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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