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Home >> Antibodies >> NDC80 Antibody / HEC1

NDC80 Antibody / HEC1 (RQ5648)

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  Catalog No Formulation Size Price (USD)  
Image RQ5648 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with NDC80 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with NDC80 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with NDC80 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HL-60 cells with NDC80 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDC80 antibody.
Flow cytometry testing of human SiHa cells with NDC80 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDC80 antibody.
Western blot testing of 1) human K562, 2) rat spleen and 3) mouse spleen lysate with NDC80 antibody. Expected molecular weight: 74-80 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O14777
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NDC80 antibody is available for research use only.
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Description

Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. It is mapped to 18p11.32. This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Application Notes

Optimal dilution of the NDC80 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids N87-E602) was used as the immunogen for the NDC80 antibody.

Storage

After reconstitution, the NDC80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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