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Home >> Antibodies >> NCF2 Antibody / NOXA2 / P67PHOX

NCF2 Antibody / NOXA2 / P67PHOX (RQ5898)

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  Catalog No Formulation Size Price (USD)  
Image RQ5898 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human tonsil with NCF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with NCF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen with NCF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen with NCF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human ThP-1 and 2) mouse RAW264.7 cell lysate with NCF2 antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of human ThP-1 cells with NCF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCF2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P19878
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NCF2 antibody is available for research use only.
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Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the NCF2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-V526) was used as the immunogen for the NCF2 antibody.

Storage

After reconstitution, the NCF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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