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Home >> Antibodies >> Napsin A Antibody

Napsin A Antibody [clone NAPSA/7165R] (V4647)

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  Catalog No Formulation Size Price (USD)  
Image V4647-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4647-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4647SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human lung adenocarcinoma tissue with NAPSA antibody (clone NAPSA/7165R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung adenocarcinoma tissue with NAPSA antibody (clone NAPSA/7165R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human brain tissue with NAPSA antibody (clone NAPSA/7165R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NAPSA/7165R
Purity Protein A affinity
UniProt O96009
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Napsin A antibody is available for research use only.
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Description

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Application Notes

Optimal dilution of the Napsin A antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 50-150) from the human protein was used as the immunogen for the Napsin A antibody.

Storage

Aliquot the Napsin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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