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Home >> Antibodies >> Myosin Phosphatase Antibody / MYPT1 / PPP1R12A

Myosin Phosphatase Antibody / MYPT1 / PPP1R12A (R30766)

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  Catalog No Formulation Size Price (USD)  
Image R30766 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) COS-7 (monkey), 5) Raji, 6) K562, 7) Caco-2 and 8) HepG2 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) COS-7 (monkey), 5) Raji, 6) K562, 7) Caco-2 and 8) HepG2 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
Western blot testing of 1) rat brain, 2) rat C6, 3) mouse liver and 4) mouse NIH3T3 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
Flow cytometry testing of human U-251 MG cells with Myosin Phosphatase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
Flow cytometry testing of human HeLa cells with Myosin Phosphatase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt O14974
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This Myosin Phosphatase antibody is available for research use only.
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Description

PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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