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Home >> Antibodies >> Myb Antibody

Myb Antibody (F48064)

  Catalog No Formulation Size Price (USD)  
Image F48064-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48064-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC analysis of FFPE human breast carcinoma tissue stained with the Myb antibody
Myb antibody western blot analysis in mouse heart tissue lysate. Predicted molecular weight ~ 72 kDa.
Availability 1-3 business days
Species Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P10242
Applications IHC (Paraffin) : 1:50-1:100
Western blot : 1:1000
Limitations This Myb antibody is available for research use only.
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Description

Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.

Application Notes

Titration of the Myb antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 506-534 from the human protein was used as the immunogen for this Myb antibody.

Storage

Aliquot the Myb antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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