MUC2 Antibody Mucin-2 CCP58 | V2197 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2197-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2197-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2197SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2197IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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MUC2 antibody CCP58 immunohistochemistry intestine

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Formalin/paraffin normal human intestine stained with MUC2 antibody (CCP58).

IHC testing of FFPE human colon carcinoma with MUC2 antibody

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	CCP58
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	4583
Localization	Cytoplasmic and cell surface
Applications	Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1) Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations	This MUC2 antibody is available for research use only.
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Description

This antibody recognizes a single glycoprotein of 520kDa, identified as MUC2 (Mucin-2). The MUC2 antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine and colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.