MUC-1 Antibody MUC1/955 | V2372 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2372-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2372-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2372SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2372IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC staining of FFPE human endometrial carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lung carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human T47D cell lysate with MUC-1 antibody (clone MUC1/955). Expected molecular weight: 120-500 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free MUC-1 antibody (clone MUC1/955) as confirmation of integrity and purity.

Western blot testing of human MCF7 cell lysate with MUC-1 antibody (clone MUC1/955). Expected molecular weight: 120-500 kDa depending on glycosylation level.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	MUC1/955
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	4582
RRID	AB_2864392
Localization	Cytoplasmic and cell surface
Applications	Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) Immunofluorescence : see citations 1 & 2
Limitations	This MUC-1 antibody is available for research use only.
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Description

Mucin-1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed in >90% breast carcinomas and metastases. The transgenic protein has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of the protein uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Mucin-1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. The specific epitope of this Mucin-1 antibody has not yet been determined.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.

>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.