• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> mtTFA Antibody / TFAM

mtTFA Antibody / TFAM (R32176)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R32176 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Bulk quote request
IF/ICC staining of FFPE human A431 cells with mtTFA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
›
IF/ICC staining of FFPE human A431 cells with mtTFA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer with mtTFA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) Jurkat, 2) A431, 3) HL-60, 4) CCRF-CEM, 5) 293T and 6) SW620 lysate with mtTFA antibody at 0.5ug/ml. Expected molecular weight 24~29 kDa.
Western blot testing of human 1) Caco-2, 2) SW620, 3) PANC-1, 4) rat brain and 5) mouse brain lysate with mtTFA antibody. Expected molecular weight 24~29 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q00059
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
IF/ICC (FFPE) : 2-4ug/ml
Limitations This mtTFA antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P, FACS
    Reactivity : Human
  • Applications : WB, IHC-P, IHC-F, ICC
    Reactivity : Human
  • Applications : IHC, FACS, IF, WB, ELISA
    Reactivity : Human
  • Applications : WB, Direct ELISA
    Reactivity : Mouse, Rat

Description

TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Application Notes

Optimal dilution of the mtTFA antibody should be determined by the researcher.

Immunogen

Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY of human mtTFA were used as the immunogen for the mtTFA antibody.

Storage

After reconstitution, the mtTFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all mtTFA antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.