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Home >> Antibodies >> MSLN Antibody / Mesothelin

MSLN Antibody / Mesothelin [clone MKPF-1] (V3821)

  Catalog No Formulation Size Price (USD)  
Image V3821-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3821-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3821SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human lung mesothelioma with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat stomach with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name MKPF-1
Purity Protein G affinity
UniProt Q13421
Localization Cytoplasmic, cell surface, secreted
Applications IHC (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This MSLN antibody is available for research use only.
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Description

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Application Notes

Optimal dilution of the MSLN antibody should be determined by the researcher.

Immunogen

A portion of amino acids 273-407 from the human protein was used as the immunogen for this MSLN antibody.

Storage

After reconstitution, the MSLN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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