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Home >> Antibodies >> MSH2 Antibody

MSH2 Antibody [clone MSH2/2622] (V7964)

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  Catalog No Formulation Size Price (USD)  
Image V7964-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7964-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7964SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human basal cell carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon from a Lynch disease patient with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human cell lysates with MSH2 antibody. Expected molecular weight: ~105 kDa.
Flow cytometry testing of permeabilized human A549 cells with MSH2 antibody; Red=isotype control, Blue= MSH2 antibody.
Immunofluorescent staining of permeabilized human MOLT-4 cells with MSH2 antibody (green) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free MSH2 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MSH2/2622
Purity Protein G affinity chromatography
UniProt P43246
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells in 0.1ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Limitations This MSH2 antibody is available for research use only.
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Description

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Application Notes

Optimal dilution of the MSH2 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 327-427) was used as the immunogen for this MSH2 antibody.

Storage

Store the MSH2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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