MRPP1 Antibody | NY-REN-49 | RQ7545 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ7545	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human ovarian cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung squamous cell carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast duct carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human ovarian cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human rectal cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human A549 cells with MRPP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HepG2, 2) Daudi and 3) HEL cell lysate with MRPP1 antibody. Predicted molecular weight ~47 kDa, commonly observed at 40-47 kDa.

Flow cytometry testing of human 293T cells with MRPP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MRPP1 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q7L0Y3
Localization	Cytoplasmic (mitochondria), nuclear
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This MRPP1 antibody is available for research use only.
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Description

The TRMT10C gene encodes 'tRNA methyltransferase 10 homolog C,' also called MRPP1 (Mitochondrial ribonuclease P protein 1) and 'Renal carcinoma antigen NY-REN-49,' the precursor of a subunit of the mitochondrial ribonuclease P, which is involved in 5' processing of mitochondrial tRNAs. The encoded protein may confer RNA-binding capacity to mitochondrial ribonuclease P and may be essential for transcript processing, RNA modification, translation and mitochondrial respiration.

Application Notes

Optimal dilution of the MRPP1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids L36-K340) was used as the immunogen for the MRPP1 antibody.

Storage

After reconstitution, the MRPP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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MRPP1 Antibody / NY-REN-49 / TRMT10C (RQ7545)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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