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Home >> Antibodies >> CD40 Antibody

CD40 Antibody (R32520)

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  Catalog No Formulation Size Price (USD)  
Image R32520 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of mouse spleen lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 32-45 kDa depending on glycosylation level.
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Western blot testing of mouse spleen lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 32-45 kDa depending on glycosylation level.
IHC testing of FFPE mouse spleen with CD40 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with CD40 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD40 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P27512
Localization Cytoplasmic, cell membrane
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
ELISA : 0.5-1ug/ml (mouse protein tested); request BSA-free format for coating
Limitations This CD40 antibody is available for research use only.
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Description

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand.

Application Notes

Differences in protocols and secondary/substrate sensitivity may require the CD40 antibody to be titrated for optimal performance.

Immunogen

Amino acids L20-R193 from the mouse protein were used as the immunogen for the CD40 antibody.

Storage

After reconstitution, the CD40 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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