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Home >> Antibodies >> MITF Antibody

MITF Antibody (F41536)

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  Catalog No Formulation Size Price (USD)  
Image F41536-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F41536-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of MITF antibody at 1:2000 dilution + A375 lysate; Predicted molecular weight: 55-60 kDa.
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Western blot testing of MITF antibody at 1:2000 dilution + A375 lysate; Predicted molecular weight: 55-60 kDa.
MITF antibody western blot analysis in K562 lysate. Predicted molecular weight: 55-60 kDa.
Confocal immunofluorescent analysis of MITF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
MITF antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram)
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O75030
Applications Western blot : 1:1000-2000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This MITF antibody is available for research use only.
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Description

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Notes

Titration of the MITF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-28 from the human protein was used as the immunogen for this MITF antibody.

Storage

Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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