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Home >> Antibodies >> METTL14 Antibody / N-Terminal Region

METTL14 Antibody / N-Terminal Region (F54217)

  Catalog No Formulation Size Price (USD)  
Image F54217-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54217-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of human 1) HL60, 2) A431, 3) Jurkat and 4) mouse NIH3T3 cell lysate with METTL14 antibody at 1:2000. Expected molecular weight: 52-65 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit Ig
Purity Peptide affinity purified
UniProt Q9HCE5
Applications Western Blot : 1:2000
Limitations This METTL14 antibody is available for research use only.
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Description

N6-adenosine-methyltransferase subunit METTL14 is an N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and self-renewal of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing and processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding developmental regulators, results in transcript destabilization, maintaining the groung state of ESCs, thereby promoting self-renewal of ESCs.

Application Notes

The stated application concentrations are suggested starting points. Titration of the METTL14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 2-36 from the N-terminal region of the human protein was used as the immunogen for the METTL14 antibody.

Storage

Aliquot the METTL14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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