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Home >> Antibodies >> Melan-A Antibody / MART-1

Melan-A Antibody / MART-1 [clone MLANA/8365R] (V5290)

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  Catalog No Formulation Size Price (USD)  
Image V5290-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5290-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5290SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human melanoma tissue with Melan-A antibody (clone MLANA/8365R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human melanoma tissue with Melan-A antibody (clone MLANA/8365R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Melan-A antibody (clone MLANA/8365R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MLANA/8365R
Purity Protein A/G affinity
UniProt Q16655
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Melan-A antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Application Notes

Optimal dilution of the Melan-A antibody should be determined by the researcher.

Immunogen

A recombinant fragment corresponding to the N-terminus of human Melan A protein was used as the immunogen for the Melan-A antibody.

Storage

Aliquot the Melan-A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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