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Home >> Antibodies >> MED9 Antibody / MED25

MED9 Antibody / MED25 (R32991)

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  Catalog No Formulation Size Price (USD)  
Image R32991 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of rat 1) brain, 2) heart, 3) kidney and moouse 4) brain, 5) heart, 6) kidney with MED9 antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
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Western blot testing of rat 1) brain, 2) heart, 3) kidney and moouse 4) brain, 5) heart, 6) kidney with MED9 antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
IHC testing of FFPE human lung cancer tissue with MED9 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with MED9 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human colon cancer tissue with MED9 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with MED9 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with MED9 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q9NWA0
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA (recombinant human protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This MED9 antibody is available for research use only.
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Description

MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through direct interactions with transcription factors. This MED9 gene is located within the Smith-Magenis syndrome region on chromosome 17.

Application Notes

Optimal dilution of the MED9 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids A55-E146 was used as the immunogen for the MED9 antibody.

Storage

After reconstitution, the MED9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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