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Home >> Antibodies >> MED8 Antibody

MED8 Antibody (R32979)

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  Catalog No Formulation Size Price (USD)  
Image R32979 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat pancreas, 2) mouse pancreas and 3) human U-2 OS lysate with MED8 antibody at 0.5ug/ml. Expected molecular weight: 29-32 kDa.
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Western blot testing of 1) rat pancreas, 2) mouse pancreas and 3) human U-2 OS lysate with MED8 antibody at 0.5ug/ml. Expected molecular weight: 29-32 kDa.
IHC testing of FFPE mouse testis tissue with MED8 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat testis tissue with MED8 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q96G25
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA (recombinant human protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This MED8 antibody is available for research use only.
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Description

Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.

Application Notes

Optimal dilution of the MED8 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids M1-R195 was used as the immunogen for the MED8 antibody.

Storage

After reconstitution, the MED8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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