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Home >> Antibodies >> MeCP2 Antibody

MeCP2 Antibody (F48100)

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  Catalog No Formulation Size Price (USD)  
Image F48100-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48100-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of MeCP2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the MeCP2 gene. Observed molecular weight: ~55 kDa and ~75 kDa.
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Western blot analysis of MeCP2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the MeCP2 gene. Observed molecular weight: ~55 kDa and ~75 kDa.
MeCP2 antibody flow cytometric analysis of MDA-MB435 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q95LG8
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This MeCP2 antibody is available for research use only.
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Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. [RefSeq].

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 399-428 from the macfa protein was used as the immunogen for this MeCP2 antibody.

Storage

Store at 4oC for up to one month. For long term, aliquot the MeCP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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