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Home >> Antibodies >> MCM7 Antibody

MCM7 Antibody [clone SPM379] (V3377)

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  Catalog No Formulation Size Price (USD)  
Image V3377-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3377-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3377SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human samples with MCM7 antibody (clone SPM379). Expected molecular weight: 80~90 kDa.
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Western blot testing of human samples with MCM7 antibody (clone SPM379). Expected molecular weight: 80~90 kDa.
IHC testing of FFPE human tonsil with MCM7 antibody (clone SPM379). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM379
Purity Protein G affinity chromatography
UniProt P33993
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This MCM7 antibody is available for research use only.
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Description

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Application Notes

Titering of the MCM7 antibody may be required for optimal performance.

Immunogen

A human full length recombinant protein was used as the immunogen for the MCM7 antibody.

Storage

Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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