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Home >> Antibodies >> MART-1 Antibody / Melan-A

MART-1 Antibody / Melan-A (V3278)

  Catalog No Formulation Size Price (USD)  
Image V3278-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3278-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3278SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human COLO-38 cell lysate with Melan-A antibody. Expected molecular weight ~20 kDa with possible doublet.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This MART-1 antibody is available for research use only.
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Description

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Application Notes

The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.

Immunogen

Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.

Storage

Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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