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Home >> Antibodies >> Recombinant MART-1 Antibody / Rabbit Monoclonal

Recombinant MART-1 Antibody / Rabbit Monoclonal [clone RMMRT1-1] (V7212)

  Catalog No Formulation Size Price (USD)  
V7212-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 379

V7212-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V7212SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 399

V7212IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 379
IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone RMMRT1-1). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone RMMRT1-1). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat. Other species not known.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, k
Clone Name RMMRT1-1
Purity Protein A affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Flow Cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant MART-1 antibody is available for research use only.

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Description

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Application Notes

The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for this recombinant MART-1 antibody.

Storage

Store the recombinant MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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