MAPKAPK2 Antibody (R36061)

Western blot testing of mouse embryonic fibroblasat lysates from double KO mice (lanes 2 & 3) and rescued by introduction of MK2 gene in second lane. MK2 / MAPKAPK2 antibody was used at 0.5ug/ml. Predicted molecular weight: ~45kDa.

Western blot of HEK293 lysate overexpressing mouse MK2-GFP or MK5-GF probed with MAPKAPK2 antibody at 0.5ug/ml: cat # R36061 on left and cat # R35113 on right. Predicted molecular weight of MAPKAPK2+GFP: ~72kDa.

Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and cat # R35113, 1.5ug used) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.