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Home >> Antibodies >> Lubricin Antibody / PRG4 / N-Terminal Region

Lubricin Antibody / PRG4 / N-Terminal Region (F54211)

  Catalog No Formulation Size Price (USD)  
Image F54211-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54211-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of human liver lysate with Lubricin antibody at 1:500. Predicted molecular weight ~151 kDa.
IF/ICC testing of fixed and permeabilized human HeLa cells with Lubricin antibody (green) at 1:25 dilution, anti-actin (red) and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit Ig
Purity Peptide affinity purified
UniProt Q92954
Localization Cytoplasmic, secreted
Applications Western Blot : 1:500
Immunofluorescence/Immunohistochemistry : 1:25
Limitations This Lubricin antibody is available for research use only.
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Description

Proteoglycan 4 (PRG4), also called Lubricin, plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Lubricin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 176-210 from the N-terminal region of the human protein was used as the immunogen for the Lubricin antibody.

Storage

Aliquot the Lubricin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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