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Home >> Antibodies >> LMAN1 Antibody / ERGIC-53

LMAN1 Antibody / ERGIC-53 (RQ4585)

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  Catalog No Formulation Size Price (USD)  
Image RQ4585 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human A431, 2) human HepG2, 3) rat liver, 4) rat heart, 5) rat C6, 6) mouse liver, 7) mouse heart and 8) mouse NIH 3T3 cell lysate with LMAN1 antibody. Predicted molecular weight ~53 kDa.
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Western blot testing of 1) human A431, 2) human HepG2, 3) rat liver, 4) rat heart, 5) rat C6, 6) mouse liver, 7) mouse heart and 8) mouse NIH 3T3 cell lysate with LMAN1 antibody. Predicted molecular weight ~53 kDa.
Western blot testing of human 1) A549, 2) HepG2, 3) HeLa and 4) 293T cell lysate with LMAN1 antibody. Predicted molecular weight ~53 kDa.
Immunofluorescent staining of FFPE human A549 cells with LMAN1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with LMAN1 antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human spleen tissue with LMAN1 antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P49257
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This LMAN1 antibody is available for research use only.
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Description

Protein ERGIC-53, also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1, is a protein that in humans is encoded by the LMAN1 gene. It is mapped to 18q21.32. The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.

Application Notes

Optimal dilution of the LMAN1 antibody should be determined by the researcher.

Immunogen

Amino acids K96-M458 from the human protein were used as the immunogen for the LMAN1 antibody.

Storage

After reconstitution, the LMAN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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