L1CAM Antibody / NCAM-L1 / CD171 (F44598)

Description

L1CAM is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Application Notes

Titration of the L1CAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. Cleavage by ADAM10 results in 180 and 40 kDa bands while cleavage by Plasmin results in 140 and 80 kDa bands.

Immunogen

A portion of amino acids 1154-1182 from the human protein was used as the immunogen for this L1CAM antibody.

Storage

Aliquot the L1CAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.