- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Ku70 + Ku80 Antibody PE Conjugate [clone KU729] (V2128PE)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V2128PE-100T | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 Tests | 519 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human and Primates. Does not react with mouse, rat and chicken. Other species not known. |
| Format | PE Conjugate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | KU729 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| Gene ID | 2547 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood Immunofluorescence : 1:50-1:100 for 30 minutes at RT |
| Limitations | This Ku70 + Ku80 antibody is available for research use only. |
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This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Store the Ku70 + Ku80 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.
ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; G22P1; Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6), ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86 Kd subunit; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; KARP1; Nuclear factor IV (NFIV); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 5 (XRCC5), Ku70 + Ku80 antibody
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