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Home >> Antibodies >> Ku70 Antibody

Ku70 Antibody (R30726)

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  Catalog No Formulation Size Price (USD)  
Image R30726 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with Ku70 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with Ku70 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Ku70 antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
ICC testing of FFPE human A549 cells. HIER: steam section in pH6 citrate buffer for 20 min.
ICC testing of FFPE human HeLa cells. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of Ku70 antibody and Lane 1: HT1080; 2: SGC; 3: A453; 4: SW620; 5: HeLa; 6: A431; 7: A549; 8: Raji cell lysate. Predicted molecular weight ~70 kDa.
Flow cytometry testing of human MCF7 cells with Ku70 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ku70 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P12956
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Ku70 antibody is available for research use only.
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Description

XRCC6 (X-Ray Repair, Complementing Defective, In Chinese Hamster, 6), also called Ku70, G22P1 or TLAA, is a protein that in humans, is encoded by the XRCC6 gene. The XRCC6 gene encodesthe subunit p70 of the p70/p80 autoantigen which consists of two proteins of molecular mass of approximately 70,000 and 80,000 daltons that dimerize to form a 10 S DNA-binding complex. XRCC6 and Mre11 are differentially expressed during meiosis. XRCC6 interacts with Baxa, a mediator of mitochondrial-dependent apoptosis. Disruption of both FANCC and XRCC6/Ku70 suppressed sensitivity to crosslinking agents, diminished chromosome breaks, and reversed defective homologous recombination. Ku70 binds directly to free DNA ends, committing them to NHEJ repair. In early meiotic prophase, however, when meiotic recombination is most probably initiated, Mre11 was abundant, whereas Ku70 was not detectable.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Ku70 (YPPDYNPEGKVTKRKH) was used as the immunogen for this Ku70 antibody.

Storage

After reconstitution, the Ku70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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