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Home >> Antibodies >> KRT20 Antibody / Cytokeratin 20 / CK20

KRT20 Antibody / Cytokeratin 20 / CK20 [clone CTKN20-1] (V7290)

  Catalog No Formulation Size Price (USD)  
Image V7290-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7290-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7290SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7290IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human HT29 cell lysate with KRT20 antibody (clone CTKN20-1). Predicted molecular weight ~46 kDa.
IHC testing of human colon with KRT20 antibody (clone CTKN20-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human; other species not tested.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CTKN20-1
Purity Protein G affinity chromatography
UniProt P35900
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This KRT20 antibody is available for research use only.
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Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. KRT20 (Cytokeratin 20, CK20) is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Application Notes

The stated application concentrations are suggested starting points. Titration of the KRT20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 196-323 from the human protein was used as the immunogen for this KRT20 antibody.

Storage

Store the KRT20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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