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Home >> Antibodies >> Keratocan Antibody / KERA

Keratocan Antibody / KERA (F54366)

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  Catalog No Formulation Size Price (USD)  
Image F54366-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54366-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of mouse eyeball lysate with Keratocan antibody. Predicted molecular weight ~41 kDa.
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Western blot testing of mouse eyeball lysate with Keratocan antibody. Predicted molecular weight ~41 kDa.
Western blot testing of human skeletal muscle lysate with Keratocan antibody. Predicted molecular weight ~41 kDa.
Western blot testing of human MDA-MB-435 cell lysate with Keratocan antibody. Predicted molecular weight ~41 kDa.
IHC testing of FFPE human skeletal muscle tissue with Keratocan antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human SK-OV-3 cells with Keratocan antibody; Blue=isotype control, Green= Keratocan antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt O60938
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Keratocan antibody is available for research use only.
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Description

The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

Application Notes

The stated application concentrations are suggested starting points. Titration of the Keratocan antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 228-257 from the human protein was used as the immunogen for the Keratocan antibody.

Storage

Aliquot the Keratocan antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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