Keratin 8 Antibody / Cytokeratin 8 [clone 3G9] (RQ4889)

Keratin 8 Antibody Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using Keratin 8 Antibody demonstrates strong green cytoplasmic and membranous staining within malignant epithelial cells. The staining pattern is consistent with expression of Cytokeratin 8 (CK8/KRT8), a type II intermediate filament protein widely expressed in simple epithelial tissues and epithelial-derived carcinomas. CK8 immunoreactivity highlights tumor-associated epithelial architecture and cellular organization, while DAPI (blue) counterstains cell nuclei. HIER: steam tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining.

Keratin 8 Antibody Breast Cancer IF. Immunofluorescent staining of FFPE human breast cancer tissue using Keratin 8 Antibody demonstrates strong green cytoplasmic and membranous staining throughout malignant epithelial cell populations. The staining pattern is consistent with expression of Cytokeratin 8 (CK8/KRT8), a type II intermediate filament protein commonly expressed in epithelial tissues and epithelial-derived carcinomas. CK8 immunoreactivity highlights tumor cell morphology, epithelial differentiation, and cytoskeletal organization, while DAPI (blue) counterstains cell nuclei. HIER: steam tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining.

Keratin 8 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE U-2 OS cells using Keratin 8 Antibody demonstrates distinct red cytoplasmic staining consistent with expression of Cytokeratin 8 (CK8/KRT8), a type II intermediate filament protein involved in epithelial cytoskeletal organization and cellular structural integrity. The staining pattern highlights the filamentous cytoplasmic distribution characteristic of intermediate filament proteins, while DAPI (blue) counterstains cell nuclei. This image demonstrates intracellular localization of CK8 and supports the utility of the antibody for immunofluorescence applications. HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.

Keratin 8 Antibody Rectal Cancer IHC. Immunohistochemical staining of FFPE human rectal cancer tissue using Keratin 8 Antibody demonstrates membranous and cytoplasmic HRP-DAB brown staining within malignant epithelial cells lining gland-forming tumor structures. The staining pattern is consistent with expression of Cytokeratin 8 (CK8/KRT8), a type II intermediate filament protein widely expressed in simple epithelial tissues and epithelial-derived carcinomas. CK8 immunoreactivity highlights neoplastic epithelial architecture and supports evaluation of epithelial differentiation in rectal cancer specimens. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.

Keratin 8 Antibody Multi-Cell Line Western Blot. Western blot analysis of placenta (lane 1), Caco-2 (lane 2), A549 (lane 3), A431 (lane 4), HeLa (lane 5), K562 (lane 6), and HEK293 (lane 7) lysates using Keratin 8 Antibody demonstrates a prominent band at approximately 56 kDa, consistent with the expected molecular weight of Cytokeratin 8 (CK8/KRT8). Cytokeratin 8 is a type II intermediate filament protein widely expressed in simple epithelial tissues and many epithelial-derived cell lines, where it contributes to cytoskeletal organization, cellular integrity, and stress-response signaling. Detection of the expected molecular weight band across multiple lysates supports expression of CK8 and the suitability of this antibody for western blot applications.

Keratin 8 Antibody A549 Cell FACS. Flow cytometry analysis of human A549 cells using Keratin 8 Antibody demonstrates a clear rightward shift of the positive cell population (blue) relative to the isotype control (green), consistent with expression of Cytokeratin 8 (CK8/KRT8). Cytokeratin 8 is a type II intermediate filament protein widely expressed in simple epithelial cells and epithelial-derived tumor cell lines, where it contributes to cytoskeletal organization and cellular structural integrity. Cells were blocked with goat serum prior to staining. Red=cells alone; Green=isotype control; Blue=Keratin 8 antibody.