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Home >> Antibodies >> Keratin 10 Antibody / Cytokeratin 10 / CK10

Keratin 10 Antibody / Cytokeratin 10 / CK10 [clone KRT10/3861] (V4475)

  Catalog No Formulation Size Price (USD)  
Image V4475-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4475-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4475SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Keratin 10 antibody (clone KRT10/3861). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
SDS-PAGE analysis of purified, BSA-free Keratin 10 antibody (clone KRT10/3861) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name KRT10/3861
Purity Protein A/G affinity
UniProt P13645
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Keratin 10 antibody is available for research use only.
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Description

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.

Application Notes

Optimal dilution of the Keratin 10 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human KRT10 protein was used as the immunogen for the Keratin 10 antibody.

Storage

Aliquot the Keratin 10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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