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Home >> Antibodies >> ITGB5 Antibody / Integrin beta 5

ITGB5 Antibody / Integrin beta 5 (RQ5891)

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  Catalog No Formulation Size Price (USD)  
Image RQ5891 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectal cancer with ITGB5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with ITGB5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with ITGB5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human A549 and 2) mouse ANA-1 lysate with ITGB5 antibody. Expected molecular weight: 88-110 kDa depending on glycosylation level.
Flow cytometry testing of human A549 cells with ITGB5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITGB5 antibody.
Flow cytometry testing of human Raji cells with ITGB5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITGB5 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P18084
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ITGB5 antibody is available for research use only.
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Description

This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.

Application Notes

Optimal dilution of the ITGB5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids S32-D689) was used as the immunogen for the ITGB5 antibody.

Storage

After reconstitution, the ITGB5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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